There is a myriad of testing and development considerations in the production of any new pharmaceutical drug. Developing accurate, effective and streamlined processes, including the use of a high throughput screening assay (HTS assay) is an important consideration for any pharmaceutical company.
One of these considerations in the development of a biochemical HTS assay is the capability of the assay selected. Some assays are a fit for HTS, others excel at inhibitor potency and selectivity profiling, while some are designed to study enzyme kinetics and residence time. Universal HTS activity assays such as Transcreener have the ability perform all of these tasks and more due to direct detection of enzyme products that can be performed in continuous (kinetic) mode.
Essential Considerations
With the customization of any high throughput screening assay, having the ability to streamline the testing process to reduce the use of reagents is often a critical factor. Transcreener HTS assays can easily be miniaturized and can be used in 96, 384, or 1536 well formats.
BellBrooks Transcreener and AptaFluor assays are designed to use direct detection methods. This affords universal measurement of nucleotides in homogenous fluorescent readouts. High throughput screening is possible by using a single set of reagents that detect the product such as ADP, across a specific enzyme family.
These assay solutions include kinase assays, phosphodiesterase assays, and ATPase assays as well as a variety of up and coming targets such as methyltransferases, glycosyltransferases, and GTPases. Each offers more than one immunoassay readout option per assay kit. These assays can be designed to screen for activators or inhibitors, further increasing the speed of completion of this phase as well as reducing the cost involved in testing.
The time required to develop an HTS assay also needs to be considered. Universal assays such as Transcreener can be used with multiple targets within a family. This feature minimizes the assay development required when testing multiple targets now and on future projects. This type of rapid turnaround and high throughput is a definitive advantage in lead discovery, SAR, and lead optimization getting the user through to the final drug development state faster, and with a higher rate of success.
At BellBrook Labs, we offer several options in high throughput screening assay kits as well as customize assay development services. To learn more, see us online at www.bellbrooklabs.com.
Tweet us at Twitter!